| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.104771 |
| Chromosome: | chromosome 7 |
| Location: | 249764 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g313850 | (1 of 1) PTHR22844//PTHR22844:SF219 - F-BOX AND WD40 DOMAIN PROTEIN // WD REPEAT-CONTAINING PROTEIN 49 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAAATGAGTGTTCTTACTGGGAAGCGCTA |
| Internal bar code: | GTAGTTTTCGTACCCAGCGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 721 |
| LEAP-Seq percent confirming: | 98.5793 |
| LEAP-Seq n confirming: | 3400 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCCGTGTGGTGACTGTGT |
| Suggested primer 2: | CACTGTCGGGAAGGTGATTT |