Insertion junction: LMJ.RY0402.104789_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.104789
Chromosome:chromosome 1
Location:3487681
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g022150 KCN3 Voltage-gated K+ channel; (1 of 1) PTHR11537//PTHR11537:SF158 - VOLTAGE-GATED POTASSIUM CHANNEL // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCACCATGCTCCAGCCCATGTGGCATGTC
Internal bar code:TCGCCGTAACGTGTGACGATAA

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:42.439
LEAP-Seq n confirming:348
LEAP-Seq n nonconfirming:472
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CCGTACCGTTCAAGTTCGTT
Suggested primer 2:GTGAGCAAGGACGTGACTGA