| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.104803 |
| Chromosome: | chromosome 17 |
| Location: | 4147348 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g729750 | NAT3,AANAT | N-acetyltransferase related to GCN5; (1 of 1) 2.3.1.87 - Aralkylamine N-acetyltransferase / Serotonin N-acetyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTACACCGCCTATGTGGCCGCTACCTCC |
| Internal bar code: | TGTTCCCGACCGGCCTCTTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 227 |
| LEAP-Seq percent confirming: | 99.0881 |
| LEAP-Seq n confirming: | 326 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCACCTGCAGTTCACACAG |
| Suggested primer 2: | CACTAGGCTCTGCCGTTTTC |