| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.104805 |
| Chromosome: | chromosome 11 |
| Location: | 83922 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467531 | FAP15 | Flagellar Associated Protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAAATAAGCCCCCATTGACAATTCCGGTC |
| Internal bar code: | TCTAGGCCCGGGGTCATCTAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 647 |
| LEAP-Seq percent confirming: | 95.7691 |
| LEAP-Seq n confirming: | 7017 |
| LEAP-Seq n nonconfirming: | 310 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTGTTTCATTCAGCGTAA |
| Suggested primer 2: | CGTGCACCGGATATGTACTG |