| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.104836 |
| Chromosome: | chromosome 13 |
| Location: | 1834322 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g575250 | (1 of 1) PTHR10663:SF114 - PROTEIN MON2 HOMOLOG | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCCGTTCGTCCAAAAGCAGGGCCAGCCG |
| Internal bar code: | GTCGTAGGCCATGTACCTCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 910 |
| LEAP-Seq percent confirming: | 99.1571 |
| LEAP-Seq n confirming: | 1647 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCGTCATTCACTTGGCAG |
| Suggested primer 2: | CACAGCCAGCAAGTACTCCA |