| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.104840 |
| Chromosome: | chromosome 8 |
| Location: | 1534099 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g364862 | (1 of 1) PF07714//PF13185 - Protein tyrosine kinase (Pkinase_Tyr) // GAF domain (GAF_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGACGCGTCAGGGAGGGTGCCGCATGGTA |
| Internal bar code: | GCTCTGCCCCTCTGGAATCCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1145 |
| LEAP-Seq percent confirming: | 99.6815 |
| LEAP-Seq n confirming: | 3756 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCTGCTGAAACAGCATCA |
| Suggested primer 2: | GTTGATGAGGAGGAGTTCGG |