| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.104849 |
| Chromosome: | chromosome 7 |
| Location: | 5859661 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g353650 | (1 of 29) PF00612 - IQ calmodulin-binding motif (IQ) | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGAGCAGAGCATATAACGGGCATGTAGAC |
| Internal bar code: | GCGCTACGGGGGCCAGATTCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 667 |
| LEAP-Seq percent confirming: | 98.8601 |
| LEAP-Seq n confirming: | 2255 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAGTGCATCGTGTACCCT |
| Suggested primer 2: | TGTACCAGCCCTGATAAGCC |