Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.104873 |
Chromosome: | chromosome_11 |
Location: | 1540969 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre11.g467753 | HMO2 | Heme oxygenase | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATCAAGGCATTCCCGCTGCCAGCCACCGCC |
Internal bar code: | TGCAGGTCTTACAAAATAGAGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 501 |
LEAP-Seq percent confirming: | 99.7459 |
LEAP-Seq n confirming: | 785 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCAACACACACAGCCTCAGT |
Suggested primer 2: | GGCCTTCAAGAAGCAGTTTG |