| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.104876 |
| Chromosome: | chromosome 13 |
| Location: | 1379397 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g571850 | APC10 | Anaphase promoting complex subunit 10; (1 of 1) K03357 - anaphase-promoting complex subunit 10 (APC10, DOC1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGAATACTGTAGCTCCTCTCCTGCCCAGT |
| Internal bar code: | AAGAGGTCCCGTCATGATTGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 910 |
| LEAP-Seq percent confirming: | 98.8576 |
| LEAP-Seq n confirming: | 2596 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATGCACTTTACCTGCTCG |
| Suggested primer 2: | CTTGCTACGTGCTTCCTTCC |