| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.104905 |
| Chromosome: | chromosome 3 |
| Location: | 628581 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145747 | SHK7,SHKH1 | (1 of 1) 4.2.3.5 - Chorismate synthase / 5-enolpyruvylshikimate-3-phosphate phospholyase; Chorismate synthase | gene_edge/mRNA_edge/3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGAGCCCCGCTTGCAGAGGCCCGCCAAAA |
| Internal bar code: | ATGGTCGTCCGTCAAAGGACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 645 |
| LEAP-Seq percent confirming: | 99.0665 |
| LEAP-Seq n confirming: | 849 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAGCAACGGGGCTCAATAC |
| Suggested primer 2: | TGTGTGTGGATATGGGAGGA |