| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.104961 |
| Chromosome: | chromosome 9 |
| Location: | 6263016 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g406416 | (1 of 1) PTHR23322:SF3 - PROTEIN SAY1 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAGCTTTGATGTATGACGTGTGTGCTTA |
| Internal bar code: | ATTCATGCGCCTCCCCTGAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 655 |
| LEAP-Seq percent confirming: | 98.5563 |
| LEAP-Seq n confirming: | 2048 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTGGCTGTGTGGATGTGT |
| Suggested primer 2: | CATGTGTTGGAGGAGGTGTG |