Insertion junction: LMJ.RY0402.104966_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.104966
Chromosome:chromosome_17
Location:4321164
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre17.g730950 KIN2-2,KHP1,FLA10 Kinesin motor protein, kinesin-2 (KRP85/95) family antisense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):GCACGTCCATACTGTACCATCCATCTGAGC
Internal bar code:TACAACGCGGGTCGGGAGCTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:726
LEAP-Seq percent confirming:0.193798
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:515
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ACCTCACTCTCCTGCCTTCA
Suggested primer 2:AGGCCTCCAATCTCGGTAGT