| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.104983 |
| Chromosome: | chromosome 9 |
| Location: | 6001671 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g402960 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTGGCACAGGGAGTTTCCGGGATGGGGG |
| Internal bar code: | TGAGTGTTATTTAAATGTGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 682 |
| LEAP-Seq percent confirming: | 97.1264 |
| LEAP-Seq n confirming: | 169 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGAGTAGTGATTGGCTGCG |
| Suggested primer 2: | AGTAGTGTGGGAGGGGTGTG |