| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.104992 |
| Chromosome: | chromosome 10 |
| Location: | 1610071 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g429550 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGCCGCAACACGCACGCCTGCCCCCGCA |
| Internal bar code: | CGGTGCGACGTAGCTTTGGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 480 |
| LEAP-Seq percent confirming: | 99.6923 |
| LEAP-Seq n confirming: | 19765 |
| LEAP-Seq n nonconfirming: | 61 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACCTCCCAGTGACTGAAT |
| Suggested primer 2: | CACCAGCGCAAAGAATATCA |