| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.105005 |
| Chromosome: | chromosome_9 |
| Location: | 5185264 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g399215 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTCCACCAGGCCTGGGGTTGGTGGGGAAAG |
| Internal bar code: | TAGATAACTAGTTTATCTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 742 |
| LEAP-Seq percent confirming: | 99.0 |
| LEAP-Seq n confirming: | 396 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGAGGAGAGCTAGTCGAA |
| Suggested primer 2: | ATCAAGGTGCCAACTTCCAC |