| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.105023 |
| Chromosome: | chromosome 3 |
| Location: | 601874 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145647 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGAGGGGCTGCACGAAGGCTGAAGATAT |
| Internal bar code: | CGACCATTGAGGAAGACAAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 420 |
| LEAP-Seq percent confirming: | 90.5451 |
| LEAP-Seq n confirming: | 1063 |
| LEAP-Seq n nonconfirming: | 111 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGTAATCTTCCGATCCAT |
| Suggested primer 2: | GCCTACTAAGCACACGCCTC |