| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.105055 |
| Chromosome: | chromosome 11 |
| Location: | 2558742 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g476150 | Glutamine cyclotransferase; (1 of 1) 2.3.2.5 - Glutaminyl-peptide cyclotransferase / Glutaminyl-tRNA cyclotransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACCAGACTGGTCTCAGTCTGCTTTGGTT |
| Internal bar code: | AGTCCAGATGCCTGTTTGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1117 |
| LEAP-Seq percent confirming: | 99.5522 |
| LEAP-Seq n confirming: | 667 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGATTCCCTGGATCAACGA |
| Suggested primer 2: | TGTGCTTTGAATGCTTCCAG |