| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.105065 |
| Chromosome: | chromosome 3 |
| Location: | 6457346 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g194750 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGCAGGATTACGTGTTTGCCAGTGTCAC |
| Internal bar code: | GAGGCCCCGCAATCGTGGGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 666 |
| LEAP-Seq percent confirming: | 99.2723 |
| LEAP-Seq n confirming: | 1910 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGTCGACACCCCATAAGC |
| Suggested primer 2: | GATTTGTCAAAACGCTGGGT |