| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.105103 |
| Chromosome: | chromosome 9 |
| Location: | 4242116 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394436 | (1 of 1) PF03030 - Inorganic H+ pyrophosphatase (H_PPase) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGGAGATGATTGCGTGTCTTGGCAGCAC |
| Internal bar code: | GCGCGCCATCTGCATGGCCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 722 |
| LEAP-Seq percent confirming: | 98.5089 |
| LEAP-Seq n confirming: | 1057 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCTTCTACAACTGCGCCC |
| Suggested primer 2: | GTATAGGCACTGCCCGTCAT |