| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.105110 |
| Chromosome: | chromosome 9 |
| Location: | 268468 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g386750 | HSP90A | Heat shock protein 90A; (1 of 1) K04079 - molecular chaperone HtpG (htpG, HSP90A) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTAGGGGTAGACACGCTCACATCAGTGG |
| Internal bar code: | TCGGCTACAGTACCGTGGACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 527 |
| LEAP-Seq percent confirming: | 98.9971 |
| LEAP-Seq n confirming: | 3751 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTGAAGAAGCGCTCTGAC |
| Suggested primer 2: | CAACGTGGTATGCCTCTCCT |