| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.105121 |
| Chromosome: | chromosome 2 |
| Location: | 8547082 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g141946 | (1 of 66) 2.7.12.1 - Dual-specificity kinase | 5'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATGTCCGGTGCCACGCTTATCGATACCA |
| Internal bar code: | TATACGACCGCGATTGAATGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 637 |
| LEAP-Seq percent confirming: | 96.5646 |
| LEAP-Seq n confirming: | 1293 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAATTGGCTTTCATGTCCT |
| Suggested primer 2: | GTACCCGCGTGAATCAAACT |