Insertion junction: LMJ.RY0402.105143_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.105143
Chromosome:chromosome_13
Location:2805308
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre13.g583000 antisense 5'UTR|outside_mRNA

Insertion site details

Flanking sequence (orientation from cassette outwards):CTTGGCAATTGTCTGTATATACACACCTCA
Internal bar code:GAACATTATTGTGATAGATTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:99.2714
LEAP-Seq n confirming:545
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TAAGGGCGTTACACAGTCCC
Suggested primer 2:ATCCAGCAGCCAACAATACC