| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.105149 |
| Chromosome: | chromosome 13 |
| Location: | 1347258 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g571500 | CST2 | Chlamydomonas-specific membrane transporter of unknown function | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGGGCTGGCCCGGAACTTCTGGAAGCTC |
| Internal bar code: | AGGACGTTTGTGCGTTGGCACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 778 |
| LEAP-Seq percent confirming: | 99.8879 |
| LEAP-Seq n confirming: | 891 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATCCAAGTCAGCTTGTCA |
| Suggested primer 2: | GCTAGCGCGGTAGTTTCATC |