| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.105168 |
| Chromosome: | chromosome 2 |
| Location: | 1228873 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g082350 | CUTA1,CUT1 | (1 of 1) K03926 - periplasmic divalent cation tolerance protein (cutA); Copper-binding protein CutA | 5'UTR|CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGCAAGAGAGCAGCCGAGGAGCGCGAGT |
| Internal bar code: | CCTCTTCCTCGCGTCGGCGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 146 |
| LEAP-Seq percent confirming: | 97.0904 |
| LEAP-Seq n confirming: | 634 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACCCCACATAGTTCTGCT |
| Suggested primer 2: | ATTGAGGCTAACAACCCACG |