Insertion junction: LMJ.RY0402.105191_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.105191
Chromosome:chromosome 14
Location:3818853
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre14.g632700 (1 of 9) 2.7.10.1 - Receptor protein-tyrosine kinase / Receptor protein tyrosine kinase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATACGGTACAACCCAACTCAACCACATTGA
Internal bar code:GGGTCTGCCTTGGAGAGCAGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:704
LEAP-Seq percent confirming:98.8795
LEAP-Seq n confirming:4324
LEAP-Seq n nonconfirming:49
LEAP-Seq n unique pos:56

Suggested primers for confirmation by PCR

Suggested primer 1:GGTTCTTTCCCATTTCCCAT
Suggested primer 2:AATTCTTGGCAATCTCGGTG