| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.105220 |
| Chromosome: | chromosome 12 |
| Location: | 5107677 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g526850 | HEL56 | (1 of 1) K14810 - ATP-dependent RNA helicase DDX56/DBP9 [EC:3.6.4.13] (DDX56, DBP9); DEAD box ATP-dependent RNA helicase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAGATGTGCGGGCAGGGGGTGGGCGGGGT |
| Internal bar code: | TGACGGGAGGTAACGAGCGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 427 |
| LEAP-Seq percent confirming: | 98.3095 |
| LEAP-Seq n confirming: | 756 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGCTGAAGGAGTACTTTG |
| Suggested primer 2: | CGTTACCTGACGTGCTTGAA |