| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.105222 |
| Chromosome: | chromosome 10 |
| Location: | 1214434 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g426400 | TRL8,TRX22 | (1 of 11) IPR012336//IPR013766 - Thioredoxin-like fold // Thioredoxin domain; Thioredoxin-like protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTGTTCCCCGTGGGCCATAGCAGCCCGA |
| Internal bar code: | GTGAAGAACAGGGGGCGGCAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 700 |
| LEAP-Seq percent confirming: | 86.062 |
| LEAP-Seq n confirming: | 3112 |
| LEAP-Seq n nonconfirming: | 504 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTCCTTGCCTGGGTACAT |
| Suggested primer 2: | ATGACTGGTGTGCTCAGTGC |