Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.105230 |
Chromosome: | chromosome_1 |
Location: | 4311680 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g029150 | DAU1,ODA7 | Outer row dynein assembly protein, predominantly cytoplasmic | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCCCTTCTAGGAAGAGCGCCTTCAAGTTGA |
Internal bar code: | ATGCGGCATAATTTAGGCGGCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 235 |
LEAP-Seq percent confirming: | 99.3431 |
LEAP-Seq n confirming: | 1966 |
LEAP-Seq n nonconfirming: | 13 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTTACAACAGGTCCGCAGGT |
Suggested primer 2: | TGCATCATGAACTCGTGGTT |