| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.105255 |
| Chromosome: | chromosome 3 |
| Location: | 740229 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g146167 | TEF10a,HHL1 | Homolog of Hypersensitive to High Light 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGAAGGCGCCGTTGATACCCGAAGGGCA |
| Internal bar code: | CCGAAGCTAGACGGGCCCTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1283 |
| LEAP-Seq percent confirming: | 99.7567 |
| LEAP-Seq n confirming: | 4510 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACTCGAGACGGTTGAGGA |
| Suggested primer 2: | GCGCCCCTATTTTCTTTAGC |