| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.105256 |
| Chromosome: | chromosome 4 |
| Location: | 2482424 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g221800 | POB2 | Proteome of basal body 2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTTCCCTTGACACGCAGTGCTGCGCAAA |
| Internal bar code: | TGGGAACGGCTGTCAACATTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 537 |
| LEAP-Seq percent confirming: | 99.2791 |
| LEAP-Seq n confirming: | 8814 |
| LEAP-Seq n nonconfirming: | 64 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCAGGGAAGTTACCGTTT |
| Suggested primer 2: | GGTTCAGACCCTACATGCGT |