| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.105269 |
| Chromosome: | chromosome 6 |
| Location: | 6422201 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g292400 | SOUL5 | (1 of 8) PF10184 - Uncharacterized conserved protein (DUF2358) (DUF2358); DUF2358 domain protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGATGTCTGACATGAGGCCGCCCAGGTTT |
| Internal bar code: | CCGGGTGGGCGGAGACGGATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 659 |
| LEAP-Seq percent confirming: | 99.1771 |
| LEAP-Seq n confirming: | 3013 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACAGGTATGCGATGGAGT |
| Suggested primer 2: | GCACCTTCCTGTGTGCCTAT |