| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.105315 |
| Chromosome: | chromosome 8 |
| Location: | 3222212 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g373550 | (1 of 1) K01515 - ADP-ribose pyrophosphatase (nudF) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTGTGTGTATGCGTCTGTGTCTGCAGCT |
| Internal bar code: | GTGACACACGCAAGACATCCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 501 |
| LEAP-Seq percent confirming: | 98.3373 |
| LEAP-Seq n confirming: | 414 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGAACCCTGAAACAGGAAC |
| Suggested primer 2: | GCGAGTACGTGTGCAAGTGT |