Insertion junction: LMJ.RY0402.105323_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.105323
Chromosome:chromosome 6
Location:3768317
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g278158 (1 of 2) IPR001283//IPR003882//IPR014044 - Cysteine-rich secretory protein, allergen V5/Tpx-1-related // Pistil-specific extensin-like protein // CAP domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGACAGCAGTGTACGTGCGGATAGCTGCA
Internal bar code:TGGTATTCATCGACAATAGGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:955
LEAP-Seq percent confirming:98.55
LEAP-Seq n confirming:3738
LEAP-Seq n nonconfirming:55
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TGGGGAGTATTGCGTTTAGG
Suggested primer 2:AACAACCGACCTATCGAACG