| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.105332 |
| Chromosome: | chromosome 4 |
| Location: | 3107867 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g225750 | (1 of 1) K18550 - IMP and pyridine-specific 5'-nucleotidase (ISN1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGAGCCCCACTCCTTACACGCCCAAGCAG |
| Internal bar code: | TCCGGTAGGTACGGTGCAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 400 |
| LEAP-Seq percent confirming: | 99.2433 |
| LEAP-Seq n confirming: | 1705 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCCACAAATCTTCAGTGC |
| Suggested primer 2: | CGACAGTATGTGTGTTGGGC |