Insertion junction: LMJ.RY0402.105350_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.105350
Chromosome:chromosome 17
Location:716498
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g701050 (1 of 1) PF08790//PF12874 - LYAR-type C2HC zinc finger (zf-LYAR) // Zinc-finger of C2H2 type (zf-met) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCGAGTCGGAGGAGGAGGAAGAGGTGCC
Internal bar code:ATCAGTGCTCGCGGGGAGGACA

Confirmation - LEAP-Seq

LEAP-Seq distance:195
LEAP-Seq percent confirming:71.7949
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ACCCAGCACATAGAACCCTG
Suggested primer 2:CTTCGTCTTCGGACGATAGC