| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.105371 |
| Chromosome: | chromosome 12 |
| Location: | 775729 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g495600 | (1 of 83) IPR029044 - Nucleotide-diphospho-sugar transferases | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGTCACCTGTGCCGCCCTGGCATCTGCAG |
| Internal bar code: | GGGCGCATGTGATAGGGAACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 433 |
| LEAP-Seq percent confirming: | 99.9225 |
| LEAP-Seq n confirming: | 1290 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGGGATATCTGGGGATAG |
| Suggested primer 2: | CACAATGTCCCGACTGTGAC |