| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.105373 |
| Chromosome: | chromosome 1 |
| Location: | 7394326 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g053200 | (1 of 1) PTHR12415:SF0 - TYROSYL-DNA PHOSPHODIESTERASE 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGACACGAATGCTACAGCTAACGTGCGC |
| Internal bar code: | TCTTAATCCCACGAATATTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 950 |
| LEAP-Seq percent confirming: | 99.7065 |
| LEAP-Seq n confirming: | 15286 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGACTTGCTTAGGTGTGCG |
| Suggested primer 2: | CGCTGCTACTGCTGGTAGTG |