| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.105374 |
| Chromosome: | chromosome 16 |
| Location: | 4368180 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g671500 | (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCCACTCCCGGCAGCGCCACCGCCACCG |
| Internal bar code: | GCCCTCTGCAAACATTTCGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 487 |
| LEAP-Seq percent confirming: | 61.6 |
| LEAP-Seq n confirming: | 154 |
| LEAP-Seq n nonconfirming: | 96 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCAGAGTCACTGACCCAC |
| Suggested primer 2: | CATCCACACCTTCTGTGTCG |