| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.105377 |
| Chromosome: | chromosome 8 |
| Location: | 1192855 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g363100 | (1 of 2) PTHR23301//PTHR23301:SF0 - CHITIN BINDING PERITROPHIN-A // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGACCAGATCTGGGACGGCGCGCTGCTG |
| Internal bar code: | AGAACTACACCGTGGCCTTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1058 |
| LEAP-Seq percent confirming: | 99.6189 |
| LEAP-Seq n confirming: | 7580 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTCATTACGGTAGCCAGA |
| Suggested primer 2: | TTGGATCAAGTTACTCCCGC |