| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.105383 |
| Chromosome: | chromosome 6 |
| Location: | 3084362 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g274650 | NUOAF4 | (1 of 3) IPR008979//IPR013857 - Galactose-binding domain-like // NADH:ubiquinone oxidoreductase intermediate-associated protein 30; Complex I intermediate-associated CIA30 protein, mitochondrial | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCCGCCTGACGTGTGACAGCTCGTTGAG |
| Internal bar code: | ACTGCGCGGGACGTACTGGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 855 |
| LEAP-Seq percent confirming: | 99.687 |
| LEAP-Seq n confirming: | 5095 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACGCTTCCTTGCTAGAAC |
| Suggested primer 2: | ACCTTCTCCTTCTTCTCCGC |