| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.105388 |
| Chromosome: | chromosome_7 |
| Location: | 2659056 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g330700 | FAP91 | Flagellar Associated Protein | sense | gene_edge/mRNA_edge/3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTTGCTTTACCGTTGCGGGCGCATGTGCCT |
| Internal bar code: | GAGCAGCGTGGCATGGCCAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 672 |
| LEAP-Seq percent confirming: | 97.8462 |
| LEAP-Seq n confirming: | 954 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCGGAGTAATTGATGATT |
| Suggested primer 2: | GCACACTTATCGAGGGTGGT |