| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.105408 |
| Chromosome: | chromosome 1 |
| Location: | 3178515 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g019900 | FAP384 | Flagellar Associated Protein 384 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCGTGTTCGTGGATGCACCACCCGCCGA |
| Internal bar code: | TGTCCGACCTATGCTAGAAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1132 |
| LEAP-Seq percent confirming: | 99.685 |
| LEAP-Seq n confirming: | 22154 |
| LEAP-Seq n nonconfirming: | 70 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACAACCCAAACCTGACTT |
| Suggested primer 2: | GCCATCCTGTCACACAACAC |