Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.105408 |
Chromosome: | chromosome 2 |
Location: | 3294472 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g095083 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGTGATGCGCCACCCTGATTAGCTAGCGC |
Internal bar code: | TCCATCCGAGTGGTGAGAAAGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 147 |
LEAP-Seq percent confirming: | 98.6091 |
LEAP-Seq n confirming: | 2765 |
LEAP-Seq n nonconfirming: | 39 |
LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAGTAACACAATGGCAGGGC |
Suggested primer 2: | TTTGGTTTAGTTTGGGCTGG |