Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.105436 |
Chromosome: | chromosome 17 |
Location: | 3984270 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre17.g728800 | IDH1 | (1 of 1) PTHR11835:SF42 - ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT BETA, MITOCHONDRIAL; Isocitrate dehydrogenase, NAD-dependent | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGTTGCGGCGGATGGAGTCCAGGACCTC |
Internal bar code: | ACAAACCCGCCGCACGCGTAGAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 464 |
LEAP-Seq percent confirming: | 67.3857 |
LEAP-Seq n confirming: | 2624 |
LEAP-Seq n nonconfirming: | 1270 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCTCTCAAGCTTCCCACTTG |
Suggested primer 2: | GGGTTTTTACACTCGGTCCA |