| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.105448 |
| Chromosome: | chromosome 7 |
| Location: | 870688 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g318600 | HSP22H | (1 of 2) PTHR11527//PTHR11527:SF42 - SMALL HEAT-SHOCK PROTEIN HSP20 FAMILY // HEAT SHOCK PROTEIN HSP-16.41-RELATED; Heat shock protein 22H | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCATTTCTTTTAGGGTGGACGAGGCGGTC |
| Internal bar code: | CGGGCAGTATCATACGTTGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 642 |
| LEAP-Seq percent confirming: | 99.746 |
| LEAP-Seq n confirming: | 1178 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAACAGAGGATGCAGTGAA |
| Suggested primer 2: | GTCTCACTTCTGTCGCCTCC |