| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.105489 |
| Chromosome: | chromosome 12 |
| Location: | 8088473 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g551600 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATCTCCATGTCTGCACGCGCGCGGTGCC |
| Internal bar code: | CACCTCCGAGCCTCACCGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 507 |
| LEAP-Seq percent confirming: | 78.6325 |
| LEAP-Seq n confirming: | 92 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGGATTGTGTGCGTGTGG |
| Suggested primer 2: | GACCTGTCGTATGCAGGGTT |