| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.105492 |
| Chromosome: | chromosome 16 |
| Location: | 2103587 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g657750 | CCD2 | Carotenoid cleavage dioxygenase; (1 of 3) IPR005404 - Potassium channel, voltage dependent, Kv3.3 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTGTGGTAGCGCGGGGCAGCAGTCATCC |
| Internal bar code: | GTTTTAACTGGTAGGCCTCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 205 |
| LEAP-Seq percent confirming: | 98.1731 |
| LEAP-Seq n confirming: | 1021 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGTTATGCACCGGACTTC |
| Suggested primer 2: | CCAGCTACGATGCAGTTGAA |