| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.105525 |
| Chromosome: | chromosome 16 |
| Location: | 5026117 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g684650 | (1 of 2) PTHR13748//PTHR13748:SF45 - COBW-RELATED // SUBFAMILY NOT NAMED; Putative metallochaperone | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTGGGGTGAAGAAGCGAAGTATGCGCCT |
| Internal bar code: | GTGTGAATTGATTCACTGCCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 444 |
| LEAP-Seq percent confirming: | 98.0732 |
| LEAP-Seq n confirming: | 1018 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAACAGTACAAATCGGGG |
| Suggested primer 2: | TGTGTGTGGATTGCGGTACT |