| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.105560 |
| Chromosome: | chromosome 3 |
| Location: | 2809215 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g162300 | SDR7 | Short-chain dehydrogenase/reductase; (1 of 13) 1.1.1.300 - NADP-retinol dehydrogenase / Retinol dehydrogenase (NADP(+)) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCCCTTCCCCCGGGTCTATAGTTCATGA |
| Internal bar code: | CCGGGTGCCGTGGGGCGGAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 33 |
| LEAP-Seq percent confirming: | 97.5369 |
| LEAP-Seq n confirming: | 1980 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACACGTCGATCTGTGTGG |
| Suggested primer 2: | CGTGTGGTGTGTGTGTGTGT |