| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.105565 |
| Chromosome: | chromosome 16 |
| Location: | 3197512 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g666302 | (1 of 20) IPR010095//IPR013083 - Transposase IS605, OrfB, C-terminal // Zinc finger, RING/FYVE/PHD-type | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGAGTTTACAGACGGACGTGGCGTAGGA |
| Internal bar code: | GGACGTGCGGCCACCCCTAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 807 |
| LEAP-Seq percent confirming: | 98.0583 |
| LEAP-Seq n confirming: | 606 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACACGAGCCCCATCTTGT |
| Suggested primer 2: | GCATCGACAGCATAGTGCAT |